Part:BBa_K199015:Design

CAT with CUAGC Addition

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 3
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

There is a mutation at the 142nd base. It mutated from an A to a G causing amino acids to change from lysine to arginine. The first two bases of the suffix are also deleted.

Source

PCR directed mutation of an existing registry part.